Gallery

a,b, VIS-GB-PAM (a) and VIS-NB-PAM (b) of a mouse brain without a skull show the depth-encoded brain vasculature. Scale bars, 1 mm. c,d, VIS-GB-PAM (c) and VIS-NB-PAM (d) for a mouse with an intact skull show the depth-encoded brain vasculature. Scale bars, 1 mm. Both of the mouse-brain vasculature images obtained using VIS-NB-PAM show more blood vessels around the edge areas compared with conventional VIS-GB-PAM. The colour scale applies to all panels.

a,b, ULM-PAM images of lipids (a) and proteins (b). T, tubular structures; N, nucleic region. c, UV-PAM image of nucleic acids. d,e, MIR-PAM images of lipids (d) and proteins (e), imaged at 3,420 nm and 6,050 nm, respectively. f, Comparison of line profiles along the dashed lines in the ULM-PAM lipid (a), ULM-PAM protein (b), MIR-PAM lipid (d) and MIR-PAM protein (e) images. g, Composite image of a cell formed by overlaying the images of lipids (a), proteins (b) and nucleic acids (c) in different colour channels. h,i, Composite images of the cells at neonatal (h) and mature (i) stages. AB, actin bundle. Scale bars, 10 µm.

3D label-free mPAM image of an unstained mouse brain embedded in a paraffin block. a A section of the entire mouse brain image (coronal view). b A 3D view of the imaged brain block corresponding to the marked region in a. c x–y image at z = 0.16 mm (coronal view), with the cell nuclei marked in blue. The yellow dashed line outlines the boundary between the cerebrum and the cerebellum. d x–z image at y = 2.31 mm (transverse view). e y–z image at x = 0.63 mm (sagittal view)

(a) Fractional photoacoustic amplitude changes (shown in yellow) in response to left hindlimb stimulation (LHS) and right hindlimb stimulation (RHS), superimposed on the vascular image (shown in red). LH, left hemisphere; RH, right hemisphere. (b) Depth-resolved photoacoustic amplitude (PA) responses. The responding areas in the LH and RH are shown in red and blue, respectively, and superimposed on the grayscale yz projection image. The signal amplitude in the yz projection image was normalized depthwise. (c) Fast sO2 imaging before (left) and during (right) stimulations of the left hindlimb. Three 0.3 × 0.3 mm2 subregions (i, ii and iii) are further analyzed (Supplementary Fig. 17). (d) Time courses of the fractional changes in the cerebral blood flow (CBF), oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO2) in the core responding region. The gray bars outline the stimulation periods. All the sO2 measurements were acquired with two lasers. The data in d are averaged over five tria

3D-PACT of human breasts in vivo. a) (top) Perspective angiogram of the right breast of a healthy human subject; (bottom) MAP image of the right breast view from the side. An imaging depth of 4 cm from the skin surface has been achieved (Supplementary Movie 3). b) Cross-sectional images of the right breast on different coronal planes from the nipple to the chest wall. Each cross-sectional image is an MAP of a 1 cm-thick slice of the breast. c-d) Left breast images of the same human subject.

SBH-PACT of healthy breasts. a) Vasculature in the right breast of a 27-year-old healthy female volunteer. Images at four depths are shown in increasing depth order from the nipple to the chest wall (also see Supplementary Movie 2). b) The same breast image with color-encoded depths. c) A close-up view of the region outlined by the magenta dashed box in b, with selected thin vessels and their line spread plots. d) A selected vessel tree with five vessel bifurcations, labeled from B1 to B5. At each bifurcation, the diameter relationships between the parent vessel (Dparent) and daughter vessels (Ddaughter) are presented on the right. X B is the junction exponent, and R B is defined as RB=D3parent/(D3daughter_a+D3daughter_b). e) Heartbeat-encoded arterial network mapping of a breast cross-sectional image (red = artery, blue = vein). f) Amplitude fluctuation in the time domain of the two pixels highlighted by yellow and green dots in e. The pixel value in the artery shows changes associated with arterial pulse pr

Label-free single impulse panoramic (SIP) PACT of small-animal whole-body anatomy from the brain to the trunk. a, Vasculature of the brain cortex. b–f, Cross-sectional images of the upper thoracic cavity (b; Supplementary Video 2), lower thoracic cavity (c; Supplementary Video 3), two lobes of the liver (d; Supplementary Video 4), upper abdominal cavity (e; Supplementary Video 5) and lower abdominal cavity (f; Supplementary Video 6). AA, abdominal aorta; BM, backbone muscles; CM, caecum; HT, heart; IN, intestines; IVC, inferior vena cava; LK, left kidney; LL, left lung; LLV, left lobe of liver; LV, liver; PV, portal vein; RK, right kidney; RL, right lung; RLV, right lobe of liver; SC, spinal cord; SP, spleen; SSS, superior sagittal sinus; ST, sternum; SV, splenic vein; TA, thoracic aorta; VE, vertebra.

Reconstructed images for breast specimen I. (a) DAS-C. (b) DAS-E1. (c) DAS-E2. (d) RCB-E2, with e = 0.5M. (e) DAS-P. (f) ARMOR-P, with e = 0.5M. (g) DAS-PP. (h) ARMOR-PP, with e = 0.5M. (i) X-ray image. (j) Inverse solution.

TAT image of the axial imaging plane. The top of represents the rear part of the head.

(a) The speckle correlation coefficient as a function of time for a living-mouse ear. Three speckle decorrelation characteristics were identified. (b) The speckle correlation curves measured at five locations on the mouse ear. The speckle correlation time (τc) determined from the curves ranged from less than 0.44–10 ms. When the blood flow was blocked, τc became much larger. (c) Schematic of the set-up for imaging an absorptive target placed between a living-mouse ear and a diffuser. The target was scanned along the x direction. Inset: a photo showing the right ear of a mouse used as a dynamic scattering medium. The left ear was bent downwards to avoid blocking the light. Aluminium foil tapes were used to block the light that did not pass through the right ear. (d) 1D images of the absorptive target. The circles and diamonds denote experimental data. The solid line denotes curve fitting of the experimental data. The dotted line denotes the four-point moving average of the experimental data. AT, absorptive tar

a, Schematic of the PAWS experimental set-up. PBS, polarized beamsplitter; SLM, spatial light modulator; λ/2, half-wave plate. b, Illustration of the two-stage optimization procedure (see Supplementary Movies 1 and 2 for more information). Stage 1: linear PAWS focuses light into the acoustic focal region. Stage 2: nonlinear PAWS focuses light onto a single-speckle grain. Blue dashed circles represent the acoustic focal region. A typical intensity distribution (green solid line) is shown above the speckle illustrations. Blue dashed envelopes represent acoustic sensitivity.

a, The probe beam scattered through the scattering medium is collected and directed to a gain module, which is composed of a gain medium and a pump source. The weak scattered photons are amplified in the gain module by stimulated emission light amplification and subsequently interfere with a reference beam to form a volume hologram inside of the PRC. b, Illustration of stimulated emission light amplification in a typical four-level gain medium, as used in our experiments; E1–E4 denote the simplified four energy levels involved in the stimulated emission light amplification. c, Reading the hologram inside of the PRC using a conjugated reference beam results in a weak time-reversed beam, which retraces its trajectory through the gain module and gains energy without change to its wavefront. After propagating through the scattering medium, the amplified time-reversed beam converges to the guidestar position. d, An example of the original time-reversed focus without the gain module, compared with the result of the

Reconstructed video shows a single laser pulse (532 nm wavelength, 7 ps pulse duration) split by a 50:50 beam splitter, imaged by T-CUP at 2.5 Tfps

First recorded video of optical mach cone captured using Compressed Ultrafast Photography (CUP)

80-Tfps imaging of a laser-induced filament in glass.

Electrical pulses in myelinated axons imaged at 20 million frames per second.

June 20, 2023. Congratulations to Peng on his graduation and Karteek on his publication!!

March 7, 2023. COIL quantum team celebrating the publications of their first set of papers in quantum imaging and physics at Caltech's Athenaeum.

December 9, 2022. COIL lab members gathered for our annual holiday party at Caltech's Moore Courtyard.

December 6, 2019. COIL lab members gathered for our annual holiday party at a local park.

February 12, 2019. AIMBE fellows at Capitol Hill advocating for federal funding for engineering and medicine. Left to right: Lihong Wang (Caltech), Shuvo Roy (UCSF), Kent Leach (UC Davis), Bruce Wheeler (UCSD), and Adam Schiff (Congressman).

December 15, 2018. COIL lab members gathered for our annual holiday party at a local restaurant.

April 27, 2017. View of the corridor leading to our research labs here at Caltech.

April 27, 2017. Group members spelling out the name of our lab 'COIL'

April 27, 2017. Group photo of the lab prior to our first Caltech open house.

December 21, 2012. Lab members, families, and special guests partying at a St. Louis restaurant.

December 19, 2009. Some lab members and families partying at the Wang residence.

December 31, 2008. Lab members in town at the New Year's Eve party at the Wang residence.

November 29, 2006. Department Chair Frank Yin (left), Lihong Wang (middle), and Chancellor Mark Wrighton at the inaugural Gene K. Beare Distinguished Chair Installation ceremony, Washington University, St. Louis, Missouri.

May 1992. Rick Smalley (left) and Lihong Wang at 1992 Rice Commencement, Houston, Texas.